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板蓝根、黄芪对猪繁殖与呼吸综合征病毒的体外抑制作用 总被引:5,自引:0,他引:5
为了解中草药黄芪、板蓝根对病毒增殖的抑制作用,对黄芪、板蓝根及二者联合使用在Marc-145的单层细胞上对猪繁殖与呼吸综合征病毒(PRRSV)增殖的抑制作用进行了研究。结果表明,板蓝根能够显著抑制PRRSV体外增殖,对PRRSV的最小直接杀灭浓度为0.195 mg/mL,最小阻断浓度为0.097 mg/mL;黄芪对PRRSV增殖的抑制作用较弱。板蓝根、黄芪联合使用时对PRRSV的抑制作用显著增强,板蓝根对PRRSV的最小直接杀灭浓度降为0.097 mg/mL,最小阻断浓度降为0.049 mg/mL。 相似文献
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In order to explore the antibacterial activity and mechanism of the Radix isatidis powder water extract,the effects of the Radix isatidis powder water extract on the morphology and structure of Escherichia coli (E.coli) were tested by scanning electron microscopy and transmission electron microscopy.The effects of the Radix isatidis powder water extract on the conductivity and total leakage rate of E.coli and the content of alkaline phosphatase in the culture medium of the content of protein,DNA and RNA were stained by DAPI to detect the effect of the Radix isatidis powder water extract on the nucleic acid of E.coli,and the effect of the Radix isatidis powder water extract on the metabolism of E.coli in vivo,in vitro,ALT,AST,pyruvic acid and ATP.The results showed that after the Radix isatidis powder water extract acted on E.coli for 10 h,it was observed by SEM that the bacteria appeared to overflow and shrink,the length of the bacteria became shorter obviously,many residues were formed due to breakage,some of them were sunken in the middle and deformed.Under TEM,it was observed that the boundary of the cell wall of E.coli was unclear,the wall membrane was zigzag,deformed and some of the bacteria were broken.The protein content outside the cell was significantly different from that in the blank control group from 8 h (P<0.01), and that in the cell from 4 h (P<0.01). DNA content had no significant difference with blank control group before 12 h (P>0.05), but had significant difference with blank control group from 16 h (P<0.01); RNA content began to decrease at 8 h, and was significantly different from blank control group at 12 h (P<0.05), and was extremely significant from 16 h (P<0.01). There was no significant difference between ALT and AST (P>0.05). The pyruvate content in culture medium and bacteria was higher than that in blank control group, and the difference was very significant from 4 h (P<0.01). The ATP content in the culture medium was significantly different from that in the blank control group (P<0.01), and the ATP content in the cell was significantly different from that in the blank control group from 4 h (P<0.01).In conclusion,the Radix isatidis powder water extract could inhibit the synthesis and metabolism of bacterial genetic material and the content of pyruvate and ATP by destroying the integrity of cell wall and cell membrane. 相似文献
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为了丰富板蓝根种质,促进板蓝根产业提质增效。采用单株选择法选育出了板蓝根新品种定蓝2号。2017 — 2019年在不同生态区进行的区域试验中, 定蓝2号鲜板蓝根折合平均产量12 787.5 kg/hm2,较对照品种定蓝1号增产5.8%。在2018 — 2019年进行的大田生产示范中,鲜板蓝根折合平均产量12 232.5 kg/hm2,较对照品种定蓝1号增产6.9%。定蓝2号特级品出成率平均22.9%,较对照品种定蓝1号提高4.0个百分点;一级品出成率平均为23.5%,较对照品种定蓝1号提高7.5个百分点。测定结果表明,定蓝2号内在质量优于2020版《中国药典》标准。定蓝2号霜霉病平均发病率为15.7%,较对照品种定蓝1号降低55.0%;病情指数为16.8,较对照品种定蓝1号降低34.1%。适宜在海拔1 800~2 300 m、年降水量400~550 mm有灌溉条件或者降水充足的地区种植。 相似文献
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3个产地板蓝根多糖作用的比较研究 总被引:6,自引:0,他引:6
通过小鼠吞噬试验、脾淋巴细胞增殖试验和最低抑菌与最低杀菌浓度的检测,对不同产地板蓝根多糖功能进行比较研究。结果表明,3个产地板蓝根多糖均使小鼠吞噬能力增强,并协同促进淋巴细胞增殖,具有一定的杀菌和抑菌效果。 相似文献
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为了建立中药材板蓝根中精氨酸的含量测定方法,采用色谱柱为ZORBAXSB-Aq(5μm,4.6×250mm),柱温30℃,以水-三氟醋酸(100∶0.2)为流动相,流速0.3mL/min;蒸发光散射检测器检测,漂移管温度110℃,载气流量2.5L/min。结果显示,线性方程为Logm=0.62630·LogA-2.29056,相关系数R=0.9996,表明精氨酸在0.2μg^1.6μg范围内线性关系良好。平均加样回收率为100.5%,RSD=1.4%(n=9)。表明该方法简便准确,重复性好。 相似文献
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[目的]明确五倍子(Rhus chinenis)、石榴皮(Folium sennae)对溶藻弧菌(Vibrio alginolyticus)及其生物膜的体外抑制作用。[方法]采用琼脂扩散法,分别测定五倍子、石榴皮对溶藻弧菌的体外抑菌作用;选用倍比稀释法确定五倍子、石榴皮对溶藻弧菌的最小抑菌浓度(MIC)和最小杀菌浓度(MBC);采用改良微孔板法评价2种中草药液对溶藻弧菌生物膜形成的影响。[结果]2种中草药都有不同程度的抑制溶藻弧菌的作用,五倍子对溶藻弧菌的抑菌圈直径为(16.37±0.14)mm,MIC和MBC均为6.25 mg/m L;石榴皮对溶藻弧菌的抑菌圈直径为(12.37±0.06)mm,MIC和MBC分别为12.50、25.00 mg/m L。当药物浓度在1.56 mg/m L及以上时,五倍子对溶藻弧菌生物膜的形成有极显著抑制作用;药物浓度在6.25 mg/m L及以上时,石榴皮对溶藻弧菌生物膜的形成有极显著抑制作用。[结论]五倍子和石榴皮对溶藻弧菌及其生物膜均有抑制作用。 相似文献
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运用主成分方法分析10个不同地区大青叶药材中的4个有效成分,提取出2个主成分;应用聚类分析对该10个不同地区的大青叶药材进行聚类分析,将各地区大青叶药材划分为5类。2种方法为大青叶药材道地性研究及鉴定提供了科学依据。 相似文献